Application of ultrasound in vinification processes

ABSTRACT

The present invention refers to a method and an equipment for the extraction of compounds from grapes by means of ultrasound in vinification processes generated through a sonoplate coupled to the walls of the pipe/duct through which the crushed grapes flow. During this extraction the transfer of phenols responsible for color from the solid portion (skin) to the liquid portion after crushing the grapes takes place as a consequence of the phenomenon known as cavitation, which allows the breaking of the skin cells and makes the phenolic compounds responsible for the color available to the liquid medium to be integrated in said liquid medium enhancing wine color.

CROSS REFERENCE TO RELATED APPLICATION

This is a divisional of U.S. application Ser. No. 15/122,810 filed onAug. 31, 2016, which was a 371 of PCT/ES2015/070130 filed on Feb. 25,2015 which claimed the priority of Spanish Patent Application No.P201430342 filed on Mar. 13, 2014, the priority of each of theseapplications is claimed and each of these applications are incorporatedherein by reference.

OBJECT OF THE INVENTION

The present invention refers to a method, a module and an equipment forthe extraction of compounds from grapes by means of ultrasound invinification processes.

In particular, the invention refers to transferring the phenoliccompounds responsible for the color from the skin to the liquid portion(must) after crushing the grapes using for this a method and anequipment based on ultrasound generation.

More particularly, the invention refers to the generation, by usingultrasound, of the phenomenon known as cavitation, which allows thebreaking of the skin cells and makes the phenolic compounds responsiblefor the color available to the liquid medium to be integrated in saidliquid medium enhancing wine color.

BACKGROUND OF THE INVENTION

The color of wine is one of the organoleptic parameters most valued bythe consumer as it provides information about its vinification,evolution and defects it may have. With the passage of time and due tooxidation processes, white wines evolve into duller hues such as goldenyellow. The same applies to rosé and red wines, the vividness of thecolors decreases over time.

Color is of special interest in red and rosé wines, due to the economicresources that must be used to extract the phenolic fraction of theskin, where phenolic compounds, those responsible for coloring, arestored. These substances affect not only the color but also contributeto the tasting phase or flavor (aroma, flavor and astringency) and alsoaffect the aftertaste or body.

Heterogeneous substances are grouped under the name of phenoliccompounds classified as follows:

Non-Flavinoid: stilbenes and phenolic acids.

Flavinoid: anthocyanins, flavanols, flavonols, flavononols and flavones.

Amongst these, the compounds that influence the color of red and roséwines the most resulting in orange, red, purple or blue colors areanthocyanins and the pigments derived from them, produced bycopigmentation or condensation processes.

The transfer of phenolic compounds responsible for the color from theskin into the liquid phase (must) after a crushing process, is closelyrelated to the raw material and vinification techniques employed and thevariety, ripeness and size of the grape affects it decisively.

The traditional maceration process or Bordeaux, starts when the grapesare crushed, but is especially effective when the alcoholic fermentationbegins. After barreling and subsequent inoculation, overpumping andpigeage are necessary such that the solid part located at the top of thetank forming the hat comes into contact with the must, increasing itscolor.

The wines produced by this process are characterized as havingrecognized quality. However, a large financial investment is requiredfor the acquisition of fitted tanks, enough space for installationthereof, personnel in charge of the process, etc. in order to carry outthe traditional maceration. Furthermore, to achieve a good productmaceration has to be developed for several days, a period of time thatsometimes the winery does not have due to mechanization in grapeharvesting.

There are alternative methods which aim to improve the yield ofmaceration, which means causing the rapid extraction of phenoliccompounds responsible for the color dramatically increasing temperature,as is the case of “flash expansion” fermentation maceration or“thermovinification”, or otherwise removing the coloring matter slowlybecause the paste remains at temperatures below 10° C., a process knownas “criomaceration”.

The process known as “flash expansion” consists of subjecting theproduced crushed grapes (grape paste) to temperatures of up to 90° C. tobe subsequently placed under vacuum. This together with rapid cooling(90° C. to 30° C. in approximately 1 sec) causes disruption of thetissues of the grape producing transfer of compounds to the must. Theflash equipment is very expensive in addition to the energy costsinvolved in the production of heat and cold. Organoleptically, the lossof aromas is a reality. As a consequence of heating, cooked aromas occurdue to an increase of compounds such as Hydroxymethylfurfural (HMF),among others.

“Thermovinification” consists of heating the whole or processed bunchesbefore alcoholic fermentation to extract their entire polyphenolicpotential. Once the grape harvest has been subjected to hightemperatures, the color will continue to be extracted during alcoholicfermentation, since the skins will remain in contact with the must-wine.The same as the previous technique, “thermovinification” is a continuousprocess, increases the yield of red grape harvest and saves on tankcapacity.

In general, wines produced with thermovinification are intended formixing with wines with better organoleptic quality but that have lowercolor intensity. These are wines where herbaceous, scorched, andaggressive aromas without freshness occur. The economic investmentrequired is high.

As for “criomaceration”, destemmed and crushed grapes are subjected forlong periods of time, about 10 days, to a temperature of 5 to 10° C. Theskins will slowly transmit phenolic compounds. The disadvantages of thistechnique are that it is not a continuous process, where the cooledharvest is stored for a period of time before being fermented. Thisrequires that the cellars are designed so that they can store largevolumes of both raw material and in-process product.

Continuous processes for extracting components from solid materials byapplication of ultrasound are also known, although these are notspecific of vinification processes. All of these are based on the use of“bar” type transducers, also called sonotrodes, as pingers; whichsonotrodes are also immersed in the product to be treated.

Thus, for example, patent application WO 2008/074072 describes anextraction process in which the product to be treated flows around asonotrode emitting high energy and low frequency ultrasonic waves(LFHP-US). Moreover, in this patent application the use of lowfrequency/high energy ultrasound is combined with heating of the productunder treatment for extraction of components. As an example, the methodused for the extraction of color from red table grapes is described,which is not a vinification process, since vinification of table grapesis absolutely prohibited, such and as reflected in COUNCIL REGULATION(EC) N° 479/2008, of 29 Apr. 2008 on the common organization of themarket in wine.

On the other hand, patent application WO 2006/099411 describes a processof peel oil extraction from citrus fruit, wherein the product to beprocessed (mixture of water and citrus peels) flows around a sonotrodeimmersed in said product, emitting high energy ultrasonic waves.

Also, Russian Patent RU 2 104 733 C1 includes the possibility ofcarrying out the extraction of solid materials from vegetable rawmaterials dissolved in ethanol, using a sonotrode immersed in saidalcoholic solution. Said vegetable raw materials are: ginseng, flowersand fruits of hawthorn, hypericum and aceria leonuri.

However, these processes characterized by using a sonotrode immersed inthe liquid phase have the problem that the performance of the sonotrodesis less than 80%. Performance means the ratio between the electricalenergy consumed and the production of ultrasonic waves, expressed in %.In the case of the sonotrodes, the transducer uses only 80% of theelectrical energy received by the generator in the production ofultrasonic waves, and the remaining 20% is used to produce heat.

Another drawback of sonotrodes is that they emit a large amount of heatduring the treatment, and since they are immersed and in contact withthe product to be treated, the heating of said liquid phase is producedwhich may lead to formation of hydroxymethylfurfural (HMF). HMF is acyclic aldehyde which is formed by the degradation of sugars, mainly bydehydration of fructose and glucose in an acid medium, especially whenthe temperature is raised for short periods of time. According to theexperiments carried out in freshly prepared grape musts, when steadilysubjected to temperatures of 65° C. for a minimum of 30 minutes, the HMFconcentration starts to increase in an accelerated manner. If thetemperature is increased, the HMF production time is drasticallyreduced, producing concentrations above 25 mg/Kg in several minutes.

An increase in the HMF concentration in the must which will besubsequently fermented produces undesirable scorched or toasted aromas.But the harmful effects it has on the health of certain rodents are evenmore worrying, since according to research conducted (1) Zhang, X. M.;Chan, C. C.; Stamp, D.; Minkin, S.; Archer, M. C.; Bruce, W. R.Initiation and promotion of colonic aberrant crypt foci in rats by5-hydroxymethyl-2-furaldehyde in thermolyzed sucrose. Carcinog. 1993,14, 773-775;2) Bakhiya, N.; Monien, B.; Frank, H.; Seidel, A.; Glatt, G.Renal organic anion transporters OAT1 and OAT3 mediate the cellularaccumulation of 5-sulfooxymethylfurfural, a reactive, nephrotoxicmetabolite of the Maillard product 5-hydroxymethylfurfural. Biochem.Pharmacol. 2009, 78, 414-419, HMF is initiator and promoter of coloncancer, nephrotoxic processes and chromosomal aberrations. However, thegreatest concern about the risk of this molecule is associated to theconversion of HMF to SMF (5-Sulfooxymethylfurfural) due to the mutagenicnature of the latter (EFSA, 2005. Opinion of the scientific panel onfood additives, flavourings, processing aids and materials in contactwith food (AFC) on a request from the commission related to flavouringgroup evaluation 13: furfuryl and furan derivatives with and withoutadditional side-chain substituents and heteroatoms from chemical group14. EFSA Journal, 2005a, 215, 1-73.). In humans, toxicity of HMF and itsderivatives is more pronounced.

Another problem with the electrodes immersed in the product to beprocessed due to the radial outward emission and despite being LFHP-US,is that the crushed grapes can buffer the ultrasound wave over distancesof a few cm, which reduces the effectiveness of the process.

DESCRIPTION OF THE INVENTION

It is an object of the present invention to provide a method, a moduleand an equipment for the extraction of compounds from grapes by means ofultrasound in vinification processes which can be carried outcontinuously, with or without recirculation, with high efficiency andavoiding undesired effects.

Another object of the present invention is to overcome existing problemsin the state of the prior art.

Other objects and additional advantages of the present invention arederived from the description made below, taking into account theenclosed figures and the example of preferred embodiment described byway of illustration but without limitation. The transducers used in themethod and installed in the module and in the equipment are of “plate”type, also referred to as sonoplates, more specifically piezoceramic ormagnetostrictive type sonoplates, depending on the desired application,since magnetostrictive sonoplates have a larger power/area coefficientthan piezoceramic sonoplates.

These are placed around a conduit, preferably a pipe narrowed at itsends, through which the crushed grapes flow coinciding with the sides ofthe polygon.

Plate type transducers are coupled to the conduit on the outsidethereof, so they are not in direct contact with the raw material to betreated.

In terms of yield, plate transducers or sonoplates are differentiatedfrom bar transducers or sonotrodes by having a higher yield of around95%. By contrast, sonotrodes are less effective having lower yields of80%. Despite this, sonoplates produce less heat energy than sonotrodes;therefore sonoplate components suffer less, thus being more robust inindustrial applications.

Each sonoplate will be able to develop a power that will range from 100to 5,000 W. The number of sonoplates per module will vary to achieveenergy intensity or power density between 0.1 W/cm³-500 W/cm³,preferably between 0.15 W/cm³ to 200 W/cm³. The amplitude of theultrasonic wave will range between 1-100 μm.

The sonoplates are arranged in modules. The power developed perultrasound module is comprised between 2 kW-10 kW, developing a powerdensity which can vary between 0.1 W/cm³-500 W/cm³, preferably between0.15 W/cm³ to 200 W/cm³. The length of each ultrasound module will varyaccording to the volume to be processed, ranging from 0.8 meters to 10meters.

Each ultrasound module comprises at least one sonoplate, at least onegenerator responsible for receiving the electrical energy andtransmitting it to the sonoplate where it is transformed into vibratingmechanical energy, which is transmitted to the crushed grapes, apolygonal pipe narrowed at its ends through which crushed grapes flowand in which the sonoplates and a structure surrounding the pipe arecoupled, acting as a protective and soundproof.

The ultrasound equipment is formed by at least one ultrasound module,pump, valves, solenoid valves, fittings and all the materials needed toestablish a closed circuit between the tank that stores crushed grapesand the ultrasound equipment. Control of the different components of theequipment may be made through a control box of “control panel” type or“PLC computer”; the operation can be performed in manual or automaticmode.

The present invention uses low frequencies to produce a more efficientextraction. The working frequency range is between 15 and 35 kHz,preferably, between 20 and 30 kHz; more preferably, between 22 kHz, and25 kHz.

The extraction of compounds from grapes, especially those that givecolor to the must/wine, is carried out dynamically by passing a variableflow rate of between 1,000 and 50,000 1/h of crushed grapes through theultrasound equipment. In order to effectively control the flow rate atwhich the crushed grapes flow, a flow meter can be installed at the endof the equipment.

In those processes in which the grape crusher does not produce asuitable liquid phase (must), installing a shredder before theultrasound equipment will be required.

The liquid fraction is very important, since the proper performance ofthe cavitation process depends on it. Cavitation consists of thesystematic production of small bubbles that tend to collide with eachother and release their energy. Said aggressive bubble collisiontogether with the associated implosion process generates wear of theskin tissue containing the phenolic compounds, these passing to theliquid fraction. Due to the cavitation process, there is an increase oftemperature of the crushed grapes being treated. The use of plate typetransducers ensures that the temperature of the process does not riseabove 50° C. thus preventing the formation of hydroxymethylfurfural(HMF).

The installation of plate type transducers reduces the production ofheat energy since 95% of the electrical energy received by thetransducer is converted into acoustic energy, and only the remaining 5%is transformed into heat. Therefore, the heat produced during thetreatment is the sum of the heat energy emitted during cavitation plus5% of heat produced directly by the sonoplate.

In the case of bar type transducers or sonotrode, heat production ishigher because only 80% of the electrical energy is converted intoacoustic energy and the remaining 20% is converted into heat.

Since the sonotrode is immersed in the product under treatment, the heatproduced by cavitation plus the heat generated by the sonotrode itselfimpact on the performance thereof; therefore the generator that supplieselectrical energy to the plate transducer in the presence of an increasein temperature, as a safety measure, reduces the power, resulting in adecrease in the production of ultrasonic waves, and therefore, a lesseffective cavitation.

In those grape harvests where the ripening is poor or for thosevarieties that contain fewer amounts of phenolic compounds in the skin,treatments are longer. Because of this time increase, the temperature ofthe crushed grapes under treatment will increase with the consequentproduction of HMF. It is for this reason that the installation of a coldexchanger is necessary in these cases to ensure low or no HMFproduction.

DESCRIPTION OF THE FIGURES

To complement the description being carried out and with the object ofhelping to a perfect understanding of the present invention, a set ofdrawings is attached as an integral part of said description, which byway of illustration and without limitation, represent the following:

FIG. 1: Block diagram of a vinification process according to theinvention

FIG. 2: General scheme of an ultrasound equipment according to theinvention

FIG. 3: Sonoplate used in the method and equipment according to theinvention.

FIG. 4: Ultrasound module according to the invention.

FIG. 5: Section of an ultrasound module according to the invention.

DESCRIPTION OF A PREFERRED EMBODIMENT

By way of example of embodiment, and by way of illustration and withoutlimitation, a method, a module and an equipment for the extraction ofcompounds from grapes by means of ultrasound in vinification processesare described below.

As seen in FIG. 1 a vinification process starts with the reception ofthe grapes and subsequent crushing thereof; the destemming can beperformed simultaneously with the crushing.

The crushed grapes pass to a tank or storage for the product undertreatment. This tank allows in addition to said storage, the operationin recirculation of the paste. The paste passes from the tank to thetreatment for color extraction by means of ultrasound (cavitation).

Once the color extraction is finished, the paste treated with ultrasoundis subjected to pressing for the separation between liquid and solidphases, where the liquid phase (must) is taken to fermentation and thesolid phase (pomace) is obtained as a residue which can be used toobtain related products.

FIG. 2 depicts a general scheme of ultrasound equipment according to theinvention that begins by pumping the product under treatment from thetank or storage. Then, and optionally, the paste can pass through ashredder or go directly to the area of treatment with ultrasound, theflow being controlled by a flow meter and, also optionally, cooling thepaste if necessary.

The different parts of the ultrasound equipment can be controlled by aPLC type control process or similar.

In an ultrasound module according to the invention, carried out by wayof example, the plate type transducers or sonoplates 1 are ofpiezoceramic type. These are shown in FIG. 3. The sonoplates areconnected to each other and to the generator, through terminals 2.

The ceramics comprising the piezoceramic type transducer have thepiezoelectric effect when its surfaces are deformed by applying electriccurrent to it, producing the acoustic wave. However, magnetostrictivetype transducers are characterized by being composed of ferromagneticmaterials; if the magnetization of a material of this type is varied thecorresponding mechanical deformation develops, and thereby the acousticwave is produced. The composition of both types of transducers alsovaries, the most commonly used material being PZT (lead zirconatetitanate), although it is not the only one, while the magnetostrictivetransducers are composed mainly of Terfenol-D (Ter=Terbium, Fe=Iron,NOL=Naval Ordenance Laboratory, D=Dysprosium).

These are positioned around a conduit formed by a hexagonal stainlesssteel pipe with a thickness of 1-8 mm comprising a narrowing at itsends.

The plate type transducers are welded to the hexagonal stainless steelpipe, but they are not in direct contact with the raw material to betreated.

Each ultrasound module 3 consists of at least one sonoplate 1, at leastone generator responsible for receiving the electrical energy andtransmitting it to the sonoplate where it is transformed into vibratingmechanical energy, which is transmitted to the crushed grapes, anhexagonal pipe narrowed at its ends, through which the crushed grapes(paste) moves and in which the sonoplates and a structure surroundingthe pipe are coupled, acting as a protective and soundproof.

As seen in FIG. 5, in this example of embodiment, the number ofsonoplates 1 per module 3 is four on each side of the polygon, that is,twenty four sonoplates 1 per module 3.

1. An ultrasound equipment for the extraction of compounds from crushedgrapes in vinification processes comprising: a conduit through which thecrushed grapes flow; at least one generator configured for transmittingelectric energy coupled to an outside of the conduit; at least onesonoplate connected to the conduit for receiving energy and configuredto directly transmit ultrasound to the crushed grapes which flow througha hexagonal pipe narrowed at its ends, without contact between thecrushed grapes and the sonoplate; and at least one sonoplate arranged oneach side of said hexagonal pipe, wherein a module develops a powerdensity between 0.1 W/cm³ and 500 W/cm³, a power comprised between 2kW-10 kW and a working frequency ranging between 15 and 35 kHz.
 2. Theultrasound equipment according to claim 1, wherein the length of eachultrasound module is comprised between 0.8 meters and 10 meters.
 3. Theultrasound equipment according to claim 1, wherein the at least onegenerator configured to transmit electric energy and the sonoplate,which produces ultrasonic wave and cavitation in the liquid phase of thecrushed grapes, result in a protective and soundproof structureconfigured to surround the pipe.
 4. The ultrasound equipment accordingto claim 1, wherein it comprises at least one ultrasound module.
 5. Theultrasound equipment according to claim 1, further comprising pumps,valves, solenoid valves, and fittings. 30
 6. The ultrasound equipmentaccording to claim 5, wherein a control box of “control panel” type or“PLC computer” controls the different components of the ultrasoundmodule and the operation can be performed in manual or automatic mode.35
 7. The ultrasound equipment according to claim 6, further comprisinga cold exchanger equipment.